XIANG, Ye-School of Medicine, Tsinghua University

Microbiology and Infectious Diseases

XIANG, Ye

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Tel: +86-10-62772587
E-mail: yxiang@tsinghua.edu.cn
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    2016-present, Associate professor, School of Medicine, Tsinghua University

    2013-2016, Assistant professor, School of Medicine, Tsinghua University

    2010-2012, Assistant research scientist, Department of Biological Sciences, Purdue University

    2005-2010, Postdoc, Department of Biological Sciences, Purdue University

    1998-2003, Ph.D candidate, Institute of Biophysics, Chinese Academy of Sciences

    1994-1998, B.S., Peking University

Dr. Xiang’s research is directed towards a molecular understanding of viruses. His laboratory mainly applies macromolecular X-ray crystallography and cryo-electron microscopy amongst other modern biophysical or biochemical techniques to understand the molecular mechanisms of viral life cycle and host anti-viral defense.

Find a way to cure viral infections

Structural biology of viruses and molecular machineries

48. Liu, Q., Ma, B.T., Qian, N.C., Zhang, F., Tan, X., Lei, J.L., Xiang, Y.* (2019). Structure of the African Swine Fever Virus major capsid protein p72. Cell Research, DOIhttps://doi.org/10.1038/s41422-019-0232-x

47. Xu, J., Wang, D., Gui, M., and Xiang, Y.* (2019). Structural assembly of the tailed bacteriophage varphi29. Nat Commun 10, 2366.

46. Xu, J., Dayan, N., Goldbourt, A.*, and Xiang, Y.* (2019). Cryo-electron microscopy structure of the filamentous bacteriophage IKe. Proc Natl Acad Sci U S A 116, 5493-5498.

45. Wang, W., Chen, S., An, B., Huang, K., Bai, T., Xu, M., Bellot, G., Ke, Y., Xiang, Y.*, and Wei, B.* (2019). Complex wireframe DNA nanostructures from simple building blocks. Nat Commun 10, 1067.

44. Wang, L., Wang, R., Wang, L., Ben, H., Yu, L., Gao, F., Shi, X., Yin, C., Zhang, F., Xiang, Y.*, Zhang L.Q.* (2019). Structural Basis for Neutralization and Protection by a Zika Virus-Specific Human Antibody. Cell Rep 26, 3360-3368 e3365.

43. Pei, Y., Wang, C., Ben, H., Wang, L., Ma, Y., Ma, Q., Xiang, Y., Zhang, L., and Liu, G. (2019). Discovery of New Hepatitis B Virus Capsid Assembly Modulators by an Optimal High-Throughput Cell-Based Assay. ACS Infect Dis 5, 778-787.

42. Huang, K., Yang, D., Tan, Z., Chen, S., Xiang, Y., Mi, Y., Mao, C., and Wei, B. (2019). Self-Assembly of Wireframe DNA Nanostructures from Junction Motifs. Angew Chem Int Ed Engl 58, 12123-12127.

41. He, M., Luo, P., Hong, J., Wang, X., Wu, H., Zhang, R., Qu, F., Xiang, Y.*, and Xu, W.* (2019). Structural Analysis of Biomolecules through a Combination of Mobility Capillary Electrophoresis and Mass Spectrometry. ACS Omega 4, 2377-2386.

40. Wang, H., Chen, X., Wang, D., Yao, C., Wang, Q., Xie, J., Shi, X., Xiang, Y., Liu, W., and Zhang, L. (2018). Epitope-focused immunogens against the CD4-binding site of HIV-1 envelope protein induce neutralizing antibodies against auto- and heterologous viruses. J Biol Chem 293, 830-846.

39. Song, W., Gui, M., Wang, X., and Xiang, Y.* (2018). Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2. PLoS Pathog 14, e1007236.

38. Gao, J., Yao, L., Xia, T., Liao, X., Zhu, D., and Xiang, Y.* (2018). Biochemistry and structural studies of kynurenine 3-monooxygenase reveal allosteric inhibition by Ro 61-8048. FASEB J 32, 2036-2045.

37. Xu, J., and Xiang, Y.* (2017). Membrane Penetration by Bacterial Viruses. J Virol 91. Invited review.

36. Miao Gui, Wenfei Song, Haixia Zhou, Jingwei Xu, Silian Chen, Ye Xiang* and Xinquan Wang*. Cryo-electron microscopy structures of the SARS-CoV spike glycoprotein reveal a prerequisite conformational state for receptor binding. Cell Res. 2017, 27(1):119-129.

35. Miao Gui, Xin Liu, Deyin Guo, Zhen Zhang, Chang-Cheng Yin*, Yu Chen*, Ye Xiang*. Electron Microscopy Studies of the Coronavirus Ribonucleoprotein Complex. Protein Cell. 2017, 8(3):219-224.

34. Xu J, Gui M, Wang D, Xiang Y*. The bacteriophage ϕ29 tail possesses a pore-forming loop for cell membrane penetration. Nature. 2016 Jun 15;534(7608):544-7.

33. Misasi J, Gilman MS, Kanekiyo M, Gui M, Cagigi A, Mulangu S, Corti D, Ledgerwood JE, Lanzavecchia A, Cunningham J, Muyembe-Tamfun JJ, Baxa U, Graham BS, Xiang Y*, Sullivan NJ*, McLellan JS. Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science. 2016 Mar 18;351(6279):1343-6.

32. Zhang Q, Gui M, Niu X, He S, Wang R, Feng Y, Kroeker A, Zuo Y, Wang H, Wang Y, Li J, Li C, Shi Y, Shi X, Gao GF, Xiang Y, Qiu X, Chen L, Zhang L. Potent neutralizing monoclonal antibodies against Ebola virus infection. Sci Rep. 2016 May 16;6:25856.

31. Sun L, Zhang X, Gao S, Rao PA, Padilla-Sanchez V, Chen Z, Sun S, Xiang Y, Subramaniam S, Rao VB, Rossmann MG (2015) Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution. Nat Commun. 6:7548. doi: 10.1038/ncomms8548.

30. Wang Z, Wu D, Liu Y, Xia X, Gong W, Qiu Y, Yang J, Zheng Y, Li J, Wang YF, Xiang Y, Hu Y, Zhou X (2015) Drosophila Dicer-2 has an RNA interference-independent function that modulates Toll immune signaling. Sci Adv. 1(9):e1500228. doi: 10.1126/sciadv.1500228.

29. Zhu DY, Wang L, Shang G, Liu X, Zhu J, Wang L, Lu DF, Kan B, Zhang JR, Xiang Y* (2014) Structural biochemistry of a Vibrio cholerae dinucleotide cyclase reveals cyclase activity regulation by folates. Mol Cell, 55:931-7.

28. Liu Y, Zhang F, Liu J, Xiao X, Zhang S, Qin C, Xiang Y, Wang P, Cheng G (2014) Transmission-blocking antibodies against mosquito C-type lectins for dengue prevention. PLoS Pathog.10:e1003931. doi:10.1371/journal.ppat.1003931.

27. Sun S, Xiang Y, Zhang XZ, Wataru A, Holdaway H, Pal P, Dimond M, Nabel G, Rossmann MG（2013） Structural analyses at pseudo atomic resolution of Chikungunya virus and antibodies show mechanisms of neutralization. elife，DOI:http://dx.doi.org/10.7554/eLife.00435. #Co-first author.

26. Zhang X, Sun S, Xiang Y, Wong J, Klose T, Raoult D, Rossmann MG. (2012) Structure of Sputnik, a virophage, at 3.5-Å resolution. PNAS, 6:18431-6.

25. Sun S, Gao S, Kondabagil K, Xiang Y, Rossmann MG, Rao VB (2012) Structure and function of the small terminase component of the DNA packaging machine in T4-like bacteriophages. PNAS, 109:817-822.

24. Zhang X, Xiang Y, Dunigan DD, Klose T, Chipman PR, Van Etten JL, Rossmann MG. (2011) Three-dimensional structure and function of the Paramecium bursaria chlorella virus capsid. PNAS, 108:14837-14842.

23. Xiang Y, Rossmann MG. (2011) Structure of bacteriophageΦ29 head fibers has a supercoiled triple repeating helix-turn-helix motif. PNAS, 108:4806-4810.

22. Xiang Y, Baxa U, Zhang Y, Steven AC, Lewis GL, Van Etten JL, Rossmann MG. (2010) Crystal structure of a virus encoded putative glycosyltransferase. J Virol. 84:12265-12273.

21. Li L, Jose J, Xiang Y, Kuhn R, Rossmann MG. (2010) Structural Changes of Envelope Proteins During Alphavirus Fusion. Nature, 468:705-708.

20. Xiang Y, Leiman P, Li L, Grime S, Anderson DL, Rossmann MG. (2009) Crystallographic insights into the autocatalytic assembly mechanism of a bacteriophage tail spike. Mol Cell. 34:375-386.

19. Cohen DN, Sham YY, Haugstad GD, Xiang Y, Rossmann MG, Anderson DL, Popham DL. (2009) Shared catalysis in virus entry and bacterial cell wall depolymerization. J Mol Biol. 387:607-18.

18. Yang N, Li DF, Feng L, Xiang Y, Liu W, Sun H, Wang DC. (2009) Structural basis for the tumor cell apoptosis-inducing activity of an antitumor lectin from the edible mushroom Agrocybe aegerita. J Mol Biol. 387:694-705.

17. Xiang Y, Morais MC, Cohen DN, Bowman VD, Anderson DL, Rossmann MG. (2008) Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophageΦ29 tail. Proc Natl Acad Sci U S A. 105:9552-9557.

16. Cohen DN, Erickson SE, Xiang Y, Rossmann MG, Anderson DL. (2008) Multifunctional roles of a bacteriophageΦ29 morphogenetic factor in assembly and infection. J Mol Biol. 378:802-815.

15. Zhang Y, Xiang Y, Van Etten JL, Rossmann MG. (2007) Structure of a glycosyltransferase involved in the glycosylation of the virus PBCV-1 major capsid protein. Structure. 15:1031-1039.

15. Xiang Y, Morais MC, Battisti AJ, Grimes S, Jardine PJ, Anderson DL, Rossmann MG. (2006) Structural changes of bacteriophageΦ29 upon DNA packaging and release. EMBO J. 25:5229-5239.

14. Xiang Y, Bosmans F, Li C, Zhang Y, Wang DC, Tytgat J. (2005) Structural basis for the voltage-gated Na+ channel selectivity of the scorpion alpha-like toxin BmK M1. J Mol Biol 353:788-803.

13. Yang N, Tong X, Xiang Y, Zhang Y, Liang Y, Sun H, Wang DC. (2005) Molecular character of the recombinant antitumor lectin from the edible mushroom Agrocybe aegerita. J Biochem (Tokyo). 138:145-150.

12. Yin L, Xiang Y, Zhu DY, Yan N, Huang RH, Zhang Y, Wang DC. (2005) Crystal structure of human SH3BGRL protein: the first structure of the human SH3BGR family representing a novel class of thioredoxin fold proteins. Proteins. 61:213-216.

11. Wang S, Li G, Xiang Y, Huang RH, Zhang Y, Wang DC. (2005) Surface morphology and kinetic properties in rapid growth of EAFP protein crystals investigated by atomic force microscopy. Acta Cryst. D61:826-831.

10. Zhu DY, Zhu YQ, Xiang Y, Wang DC. (2005) Optimizing protein crystal growth through dynamic seeding. Acta Cryst. D61:772-775.

9. Zhu DY, Zhu YQ, Huang RH, Xiang Y, Yang N, Lu HX, Li GP, Jin Q, Wang DC. (2005) Crystal structure of the copper homeostasis protein (CutCm) from Shigella flexneri at 1.7 Å resolution: the first structure of a new sequence family of TIM barrels. Proteins. 58:764-768.

8. Li C, Guan RJ, Xiang Y, Zhang Y, Wang DC. (2004) Structure of an excitatory insect-specific toxin with an analgesic effect on mammals from the scorpion Buthus martensii Karsch. Acta Cryst. D61:14-21.

7. Xiang Y, Huang RH, Liu XZ, Zhang Y, Wang DC. (2004) Crystal structure of a novel antifungal protein distinct with five disulfide bridges from Eucommia ulmoides Oliver at an atomic resolution. J Struct Biol. 148:86-97.

6. Guan RJ, Xiang Y, He XL, Wang CG, Wang M, Zhang Y, Sundberg EJ, Wang DC. (2004) Structural mechanism governing cis and trans isomeric states and an intramolecular switch for cis/trans isomerization of a non-proline peptide bond observed in crystal structures of scorpion toxins. J Mol Biol. 341:1189-1204. #equally contributed first author

5. Huang RH, Xiang Y, Tu GZ, Zhang Y, Wang DC. (2004) Solution structure of Eucommia antifungal peptide: a novel structural model distinct with a five-disulfide motif. Biochemistry. 43:6005-6012.

4. Wang S, Xiang Y, Li GP & Wang DC (2003) Fast growth rates of Eucommia antifungal protein (EAFP) crystals observed by atomic force microscope. Progress in Biochemistry and Biophysics. 30:784-791.

3. Huang RH, Xiang Y, Liu XZ, Zhang Y, Hu Z, Wang DC. (2002) Two novel antifungal peptides distinct with a five-disulfide motif from the bark of Eucommia ulmoides Oliver. FEBS Lett. 521:87-90.

2. Xiang Y, Huang, RH, Liu W, Li, GP, Liu, XZ & Wang DC (2002) Crystallization and preliminary crystallographic studies of a novel antifungal protein with five disulfide bridges from Eucommia ulmoides Oliver. Acta Cryst. D58:1838-1840.

1. Li GP, Xiang Y, Zhang Y & Wang DC. (2001) A simple and efficient innovation of the vapor-diffusion method for controlling nucleation and growth of large protein crystals. J. Appl. Cryst. 34:388-391.