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生物成像技术讲座通知 时间:2018-07-25 16:14:32.0

生物成像技术讲座通知

结构生物学高精尖创新中心及生物医学工程系邀请北京大学李毓龙博士于7月30日14:00在生物技术馆2201做关于神经递质荧光探针的报告李博士毕业于北京大学生命科学学院,于2006年获美国杜克大学神经生物学博士,现为北京大学生命科学学院及麦戈文脑研究所研究员。李博士现在的工作主要集中在成像工具的开发,包括光遗传学、荧光成像、遗传编码的检测神经递质的荧光探针,以及利用这些工具进行功能性和生理性的研究, 包括新型小分子神经递质、神经肽、嗅觉传导或睡眠过程中脑的工作机制等。李博士有关检测多巴胺和乙酰胆碱的荧光探针的工作近期分别发表在Cell和Nature Biotechnology.

讲座时间:2018年7月30日 14:00-15:00

地点:生物技术馆2201

主讲人:Dr. Yuan Kexin

报名方式:010-62789416-171/215   zhangzehui@biomed.tsinghua.edu.cn

邮件注明:姓名、课题组、手机号、学号、院系

注:请于7月29日下午17:00 前报名

有兴趣与李毓龙博士面谈的老师请联系苑克鑫 kexinyuan@mail.tsinghua.edu.cn

 

A Genetically Encoded Fluorescent Sensor Enables Rapid and Specific Detection of Dopamine in Flies, Fish, and Mice

Yulong Li

School of Life Sciences and McGovern Brain InstitutePeking University

        Dopamine (DA) is a central monoamine neurotransmitter involved in many physiological and pathological processes. A longstanding yet largely unmet goal is to measure DA changes reliably and specifically with high spatiotemporal precision, particularly in animals executing complex behaviors. Here, we report the development of genetically encoded GPCR-activation-based-DA (GRABDA) sensors that enable these measurements. In response to extracellular DA, GRABDA sensors exhibit large fluorescence increases (ΔF/F0 90%) with subcellular resolution, subsecond kinetics, nanomolar to submicromolar affinities, and excellent molecular specificity. GRABDA sensors can resolve a single-electrical-stimulus-evoked DA release in mouse brain slices and detect endogenous DA release in living flies, fish, and mice. In freely behaving mice, GRABDA sensors readily report optogenetically elicited nigrostriatal DA release and depict dynamic mesoaccumbens DA signaling during Pavlovian conditioning or during sexual behaviors. Thus, GRABDA sensors enable spatiotemporally precise measurements of DA dynamics in a variety of model organisms while exhibiting complex behaviors.


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